Retinal ganglion cells: GABA/GLY/GLU

Tissue: Rabbit retina / Ganglion cell layer

Fixation: 250 mM glutaraldehdye, 333 mM paraformaldehyde in 0.1 M phosphate buffer, 3% sucrose

Format: 3 registered, serial, 250 nm deplasticized resin sections

Probes: GABA=RED GLYCINE=GREEN GLUTAMATE=BLUE

Caption: All neurons in the ganglion cell layer (GCL) possess a stereotypic small molecule signature.
GABA / GLYCINE / GLUTAMATE signals differentiate many subtypes of neurons in the GCL including
blue pure glutamate ganglion cells (GCs), red high GABA amacrine cells, bright magenta GABA+ GCs,
dim magenta GCs gap-junction coupled to various GABAergic ACs, dim cyan GCs gap-junction coupled
to glycinergic ACs. The black background is comprised almost entirely of glial Müller's cell
processes that lack any significant signals.

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