Retina: 7 channel metabolic phenotyping with 40 nm sections
Retinal ganglion cells: GABA/GLY/GLU
| Tissue: Rabbit retina / vertical 40 nm sections - 7 channel image |
| Fixation: 250 mM glutaraldehdye, 333 mM paraformaldehyde in 0.1 M phosphate buffer, 3% sucrose |
| Format: 7 registered, serial, 40 nm deplasticized resin sections |
| Probes: ASP=magenta GLU=blue GLY=cyan GTH=green GLN=yellow TAU=orange GABA=RED |
| Caption: Metabolic phenotyping by 7 channel imaging of registered 40 nm sections One of the novel of features high-performance immunocytochemistry is the fact that signal strength is independent of section thickness, thus enabling use of thinner sections. A 40 nm section is thinner than practical for most electron microscopy and over 1/5 as thin as the average Z-axis step of confocal microscopy. This series of seven 40 nm sections encompasses less than 0.3 microns of tissue thickness and the same structures at every point in the image are probed for all seven metabolites. |
| Abbreviations: AC(s): amacrine cell(s) BC(s): bipolar cell(s) GC(s): ganglion cell(s) GCL: ganglion cell layer HC(s): horizontal cell(s) ILM: inner limiting membrane INL: inner nuclear layer IPL: inner plexiform layer MC: Müller cell processes OFL: optic fiber layer ONL: outer nuclear layer OPL: outer plexiform layer RL: photoreceptor inner/outer segment layer VE: vascular endothelium |
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